Phosphate Buffered Saline Recipe 10X For Lab Use

Are you looking to prepare your own Phosphate Buffered Saline (PBS) solution at home? PBS is a widely used buffer solution in biological research, especially for cell culture and molecular biology applications. This simple recipe will guide you through the preparation process and provide you with a detailed list of ingredients. Let’s dive into it!

Image of PBS Solution

This image showcases a typical PBS solution, perfect for your laboratory needs.

Ingredients for Phosphate Buffered Saline (1X)

To prepare your PBS solution, you’ll need the following ingredients:

0.2 g of sodium chloride (NaCl)
0.01 g of potassium chloride (KCl)
0.02 g of disodium phosphate (Na₂HPO₄)
0.01 g of potassium dihydrogen phosphate (KH₂PO₄)
1 liter of distilled water

Instructions for Preparing PBS (1X)

Follow these simple steps to create your own Phosphate Buffered Saline:

Start by measuring out the sodium chloride, potassium chloride, disodium phosphate, and potassium dihydrogen phosphate using an accurate scale.
Combine all measured powders into a clean beaker or flask.
Add approximately 900 mL of distilled water to the container with the powders. Stir the mixture gently until all the solids are completely dissolved.
Once everything is dissolved, add additional distilled water to bring the total volume of the solution to 1 liter.
Adjust the pH of the solution to about 7.4, if necessary. You can use a pH meter or pH strips for accuracy.
Transfer the final solution into sterilized bottles or containers and store them at room temperature or in the refrigerator for longer shelf life.

Your homemade PBS solution is now ready for use in various lab procedures. Not only is PBS a versatile buffer, but it’s also crucial for maintaining the stability of your experiments. Enjoy your research and happy experiments!